Abstracto
- The Plackett-Burman statistical design was initially applied for Screening the most important nutritional variables (four carbon sources, two nitrogen sources, K2HPO4 y MgSO4 and a trace elements solution) affecting microorganism biomass production. Subsequently, a optimising the process by applied a Box-Behnken design for the most important factors tree. The results showed that biomass (UFC/mL) production was strongly affected by nutritional and physico conditions of culture medium. The highest yield was obtained in the following conditions: 3g/L sucrose, 6,75 mL/L molasses, 0,77 mL/L glycerol, 14,42 mL/L soybean hydrolyzate, 0,848 g/L yeast hidrolyzate, 0,25 g/L K2HPO4, 3/5 aeration and shaking at 120 rpm 26 hours total fermentation time during which 1 x 109 UFC/mL were obtained.
